pDae079 (eMutaT7[transition]

LOCUS       pDae079 (eMutaT7[transition]               12662 bp ds-DNA     circular SYN 02-AUG-2022
DEFINITION  Expresses eMutaT7[TadA-8e], eMutaT7[PmCDA1] and UGI for mutagenizing
            genes downstream from a T7 promoter in e.coli.
ACCESSION   .
VERSION     .
KEYWORDS    pDae079 (eMutaT7[transition])
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12662)
  AUTHORS   Seo D, Eom G, Kim HW, Kim S
  TITLE     A dual gene-specific mutator system installs all transition 
            mutations at similar rates in vivo
  JOURNAL   bioRxiv June 2022
REFERENCE   2  (bases 1 to 12662)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 2, 2022 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..12662
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(96..974)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1001..1285
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     promoter        4673..4957
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     CDS             8403..8657
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="MTNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHT
                     AYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     terminator      8866..8952
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      9044..9071
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        9090..9181
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9639..10094
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9726..9745)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9936..9957
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(10650..11309)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     11224..11243
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(11310..11412)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(11804..11821)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11938..12483)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(12018..12037)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12559..12578
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac
       61 tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca
      121 ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta
      181 aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata
      241 ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag
      301 cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag
      361 caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg
      421 tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct
      481 tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc
      541 ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc
      601 gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca
      661 tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga
      721 tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa
      781 acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata
      841 taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc
      901 ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt
      961 tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat
     1021 tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta
     1081 accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt
     1141 aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca
     1201 ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta
     1261 tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcgaatt cgagctcagg
     1321 aggaattcac catggcatat gagtgaagtt gaattcagcc atgaatattg gatgcgtcat
     1381 gccctgaccc tggcaaaacg tgcccgcgat gaacgtgaag tgccggttgg tgcggtgctg
     1441 gttttaaata atcgtgtgat tggcgaaggc tggaatcgtg cgattggtct gcatgatcct
     1501 accgcacatg cagaaatcat ggccctgcgt cagggtggtc tggttatgca aaattatcgc
     1561 ctgattgatg ccaccctgta tgtgaccttt gaaccttgtg tgatgtgtgc gggtgcaatg
     1621 attcacagtc gtattggccg tgttgtgttt ggcgttcgta atagcaagcg cggtgccgcc
     1681 ggcagcctga tgaatgttct gaattatcct ggcatgaatc atcgcgtgga aattaccgag
     1741 ggtattctgg ccgatgaatg tgccgcactg ctgtgcgatt tttatcgcat gccgcgtcaa
     1801 gtgtttaatg cacaaaagaa agcacagagc agcattaata gcggcagcga gactcccggg
     1861 acctcagagt ccgccacacc cgaaagtaac acgattaaca tcgctaagaa cgacttctct
     1921 gacatcgaac tggctgctat cccgttcaac actctggctg accattacgg tgagcgttta
     1981 gctcgcgaac agttggccct tgagcatgag tcttacgaga tgggtgaagc acgcttccgc
     2041 aagatgtttg agcgtcaact taaagctggt gaggttgcgg ataacgctgc cgccaagcct
     2101 ctcatcacta ccctactccc taagatgatt gcacgcatca acgactggtt tgaggaagtg
     2161 aaagctaagc gcggcaagcg cccgacagcc ttccagttcc tgcaagaaat caagccggaa
     2221 gccgtagcgt acatcaccat taagaccact ctggcttgcc taaccagtgc tgacaataca
     2281 accgttcagg ctgtagcaag cgcaatcggt cgggccattg aggacgaggc tcgcttcggt
     2341 cgtatccgtg accttgaagc taagcacttc aagaaaaacg ttgaggaaca actcaacaag
     2401 cgcgtagggc acgtctacaa gaaagcattt atgcaagttg tcgaggctga catgctctct
     2461 aagggtctac tcggtggcga ggcgtggtct tcgtggcata aggaagactc tattcatgta
     2521 ggagtacgct gcatcgagat gctcattgag tcaaccggaa tggttagctt acaccgccaa
     2581 aatgctggcg tagtaggtca agactctgag actatcgaac tcgcacctga atacgctgag
     2641 gctatcgcaa cccgtgcagg tgcgctggct ggcatctctc cgatgttcca accttgcgta
     2701 gttcctccta agccgtggac tggcattact ggtggtggct attgggctaa cggtcgtcgt
     2761 cctctggcgc tggtgcgtac tcacagtaag aaagcactga tgcgctacga agacgtttac
     2821 atgcctgagg tgtacaaagc gattaacatt gcgcaaaaca ccgcatggaa aatcaacaag
     2881 aaagtcctag cggtcgccaa cgtaatcacc aagtggaagc attgtccggt cgaggacatc
     2941 cctgcgattg agcgtgaaga actcccgatg aaaccggaag acatcgacat gaatcctgag
     3001 gctctcaccg cgtggaaacg tgctgccgct gctgtgtacc gcaaggacaa ggctcgcaag
     3061 tctcgccgta tcagccttga gttcatgctt gagcaagcca ataagtttgc taaccataag
     3121 gccatctggt tcccttacaa catggactgg cgcggtcgtg tttacgctgt gtcaatgttc
     3181 aacccgcaag gtaacgatat gaccaaagga ctgcttacgc tggcgaaagg taaaccaatc
     3241 ggtaaggaag gttactactg gctgaaaatc cacggtgcaa actgtgcggg tgtcgataag
     3301 gttccgttcc ctgagcgcat caagttcatt gaggaaaacc acgagaacat catggcttgc
     3361 gctaagtctc cactggagaa cacttggtgg gctgagcaag attctccgtt ctgcttcctt
     3421 gcgttctgct ttgagtacgc tggggtacag caccacggcc tgagctataa ctgctccctt
     3481 ccgctggcgt ttgacgggtc ttgctctggc atccagcact tctccgcgat gctccgagat
     3541 gaggtaggtg gtcgcgcggt taacttgctt cctagtgaaa ccgttcagga catctacggg
     3601 attgttgcta agaaagtcaa cgagattcta caagcagacg caatcaatgg gaccgataac
     3661 gaagtagtta ccgtgaccga tgagaacact ggtgaaatct ctgagaaagt caagctgggc
     3721 actaaggcac tggctggtca atggctggct tacggtgtta ctcgcagtgt gactaagcgt
     3781 tcagtcatga cgctggctta cgggtccaaa gagttcggct tccgtcaaca agtgctggaa
     3841 gataccattc agccagctat tgattccggc aagggtctga tgttcactca gccgaatcag
     3901 gctgctggat acatggctaa gctgatttgg gaatctgtga gcgtgacggt ggtagctgcg
     3961 gttgaagcaa tgaactggct taagtctgct gctaagctgc tggctgctga ggtcaaagat
     4021 aagaagactg gagagattct tcgcaagcgt tgcgctgtgc attgggtaac tcctgatggt
     4081 ttccctgtgt ggcaggaata caagaagcct attcagacgc gcttgaacct gatgttcctc
     4141 ggtcagttcc gcttacagcc taccattaac accaacaaag atagcgagat tgatgcacac
     4201 aaacaggagt ctggtatcgc tcctaacttt gtacacagcc aagacggtag ccaccttcgt
     4261 aagactgtag tgtgggcaca cgagaagtac ggaatcgaat cttttgcact gattcacgac
     4321 tccttcggta ccattccggc tgacgctgcg aacctgttca aagcagtgcg cgaaactatg
     4381 gttgacacct atgagtcttg tgatgtactg gctgatttct acgaccagtt cgctgaccag
     4441 ttgcacgagt ctcaattgga caaaatgcca gcacttccgg ctaaaggtaa cttgaacctc
     4501 cgtgacatct tagagtcgga cttcgcgttc gcgtaactgc agtataagct tggctgtttt
     4561 ggcggatgag agaagatttt cagcctgata cagattaaat cagaacgcag aagcggtctg
     4621 ataaaacaga atttgcctgg cggcagactt ttcatactcc cgccattcag agaagaaacc
     4681 aattgtccat attgcatcag acattgccgt cactgcgtct tttactggct cttctcgcta
     4741 accaaaccgg taaccccgct tattaaaagc attctgtaac aaagcgggac caaagccatg
     4801 acaaaaacgc gtaacaaaag tgtctataat cacggcagaa aagtccacat tgattatttg
     4861 cacggcgtca cactttgcta tgccatagca tttttatcca taagattagc ggatcctacc
     4921 tgacgctttt tatcgcaact ctctactgtt tctccatacc cgtttttttg ggctagcgaa
     4981 ttcgagctca ggaggaattc accatggcat tatcatatga tgacagacgc cgagtacgtg
     5041 cgcattcatg agaaactgga tatttacacc ttcaagaagc agttcttcaa caacaagaaa
     5101 tctgtgtcac accgctgcta cgtgctgttt gagttgaagc gaaggggcga aagaagggct
     5161 tgcttttggg gctatgccgt caacaagccc caaagtggca ccgagagagg aatacacgct
     5221 gagatattca gtatccgaaa ggtggaagag tatcttcggg ataatcctgg gcagtttacg
     5281 atcaactggt attccagctg gagtccttgc gctgattgtg ccgagaaaat tctggaatgg
     5341 tataatcagg aacttcgggg aaacgggcac acattgaaaa tctgggcctg caagctgtac
     5401 tacgagaaga atgcccggaa ccagatagga ctctggaatc tgagggacaa tggtgtaggc
     5461 ctgaacgtga tggtttccga gcactatcag tgttgtcgga agattttcat ccaaagctct
     5521 cataaccagc tcaatgaaaa ccgctggttg gagaaaacac tgaaacgtgc ggagaagtgg
     5581 agatccgagc tgagcatcat gatccaggtc aagattctgc ataccactaa gtctccagcc
     5641 gttagcgaga ctcccgggac ctcagagtcc gccacacccg aaagtaacac gattaacatc
     5701 gctaagaacg acttctctga catcgaactg gctgctatcc cgttcaacac tctggctgac
     5761 cattacggtg agcgtttagc tcgcgaacag ttggcccttg agcatgagtc ttacgagatg
     5821 ggtgaagcac gcttccgcaa gatgtttgag cgtcaactta aagctggtga ggttgcggat
     5881 aacgctgccg ccaagcctct catcactacc ctactcccta agatgattgc acgcatcaac
     5941 gactggtttg aggaagtgaa agctaagcgc ggcaagcgcc cgacagcctt ccagttcctg
     6001 caagaaatca agccggaagc cgtagcgtac atcaccatta agaccactct ggcttgccta
     6061 accagtgctg acaatacaac cgttcaggct gtagcaagcg caatcggtcg ggccattgag
     6121 gacgaggctc gcttcggtcg tatccgtgac cttgaagcta agcacttcaa gaaaaacgtt
     6181 gaggaacaac tcaacaagcg cgtagggcac gtctacaaga aagcatttat gcaagttgtc
     6241 gaggctgaca tgctctctaa gggtctactc ggtggcgagg cgtggtcttc gtggcataag
     6301 gaagactcta ttcatgtagg agtacgctgc atcgagatgc tcattgagtc aaccggaatg
     6361 gttagcttac accgccaaaa tgctggcgta gtaggtcaag actctgagac tatcgaactc
     6421 gcacctgaat acgctgaggc tatcgcaacc cgtgcaggtg cgctggctgg catctctccg
     6481 atgttccaac cttgcgtagt tcctcctaag ccgtggactg gcattactgg tggtggctat
     6541 tgggctaacg gtcgtcgtcc tctggcgctg gtgcgtactc acagtaagaa agcactgatg
     6601 cgctacgaag acgtttacat gcctgaggtg tacaaagcga ttaacattgc gcaaaacacc
     6661 gcatggaaaa tcaacaagaa agtcctagcg gtcgccaacg taatcaccaa gtggaagcat
     6721 tgtccggtcg aggacatccc tgcgattgag cgtgaagaac tcccgatgaa accggaagac
     6781 atcgacatga atcctgaggc tctcaccgcg tggaaacgtg ctgccgctgc tgtgtaccgc
     6841 aaggacaagg ctcgcaagtc tcgccgtatc agccttgagt tcatgcttga gcaagccaat
     6901 aagtttgcta accataaggc catctggttc ccttacaaca tggactggcg cggtcgtgtt
     6961 tacgctgtgt caatgttcaa cccgcaaggt aacgatatga ccaaaggact gcttacgctg
     7021 gcgaaaggta aaccaatcgg taaggaaggt tactactggc tgaaaatcca cggtgcaaac
     7081 tgtgcgggtg tcgataaggt tccgttccct gagcgcatca agttcattga ggaaaaccac
     7141 gagaacatca tggcttgcgc taagtctcca ctggagaaca cttggtgggc tgagcaagat
     7201 tctccgttct gcttccttgc gttctgcttt gagtacgctg gggtacagca ccacggcctg
     7261 agctataact gctcccttcc gctggcgttt gacgggtctt gctctggcat ccagcacttc
     7321 tccgcgatgc tccgagatga ggtaggtggt cgcgcggtta acttgcttcc tagtgaaacc
     7381 gttcaggaca tctacgggat tgttgctaag aaagtcaacg agattctaca agcagacgca
     7441 atcaatggga ccgataacga agtagttacc gtgaccgatg agaacactgg tgaaatctct
     7501 gagaaagtca agctgggcac taaggcactg gctggtcaat ggctggctta cggtgttact
     7561 cgcagtgtga ctaagcgttc agtcatgacg ctggcttacg ggtccaaaga gttcggcttc
     7621 cgtcaacaag tgctggaaga taccattcag ccagctattg attccggcaa gggtctgatg
     7681 ttcactcagc cgaatcaggc tgctggatac atggctaagc tgatttggga atctgtgagc
     7741 gtgacggtgg tagctgcggt tgaagcaatg aactggctta agtctgctgc taagctgctg
     7801 gctgctgagg tcaaagataa gaagactgga gagattcttc gcaagcgttg cgctgtgcat
     7861 tgggtaactc ctgatggttt ccctgtgtgg caggaataca agaagcctat tcagacgcgc
     7921 ttgaacctga tgttcctcgg tcagttccgc ttacagccta ccattaacac caacaaagat
     7981 agcgagattg atgcacacaa acaggagtct ggtatcgctc ctaactttgt acacagccaa
     8041 gacggtagcc accttcgtaa gactgtagtg tgggcacacg agaagtacgg aatcgaatct
     8101 tttgcactga ttcacgactc cttcggtacc attccggctg acgctgcgaa cctgttcaaa
     8161 gcagtgcgcg aaactatggt tgacacctat gagtcttgtg atgtactggc tgatttctac
     8221 gaccagttcg ctgaccagtt gcacgagtct caattggaca aaatgccagc acttccggct
     8281 aaaggtaact tgaacctccg tgacatctta gagtcggact tcgcgttcgc gtaactgcag
     8341 cccggggatc ctaacagagc gcgctctgtt tgagtactag caataaataa ggaggatttt
     8401 ttatgaccaa cctttccgac atcatagaga aggaaacagg caaacagttg gtcatccaag
     8461 agtcgatact catgcttcct gaagaagttg aggaggtcat tgggaataag ccggaaagtg
     8521 acattctcgt acacactgcg tatgatgaga gcaccgatga gaacgtgatg ctgctcacgt
     8581 cagatgcccc agagtacaaa ccctgggctc tggtgattca ggactctaat ggagagaaca
     8641 agatcaagat gctgtaaaag cttggctgtt ttggcggatg agagaagatt ttcagcctga
     8701 tacagattaa atcagaacgc agaagcggtc tgataaaaca gaatttgcct ggcggcagta
     8761 gcgcggtggt cccacctgac cccatgccga actcagaagt gaaacgccgt agcgccgatg
     8821 gtagtgtggg gtctccccat gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag
     8881 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
     8941 agtaggacaa atccgccggg agcggatttg aacgttgcga agcaacggcc cggagggtgg
     9001 cgggcaggac gcccgccata aactgccagg catcaaatta agcagaaggc catcctgacg
     9061 gatggccttt ttgcgtttct acaaactctt ttgtttattt ttctaaatac attcaaatat
     9121 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     9181 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     9241 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     9301 agcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     9361 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     9421 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     9481 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     9541 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     9601 tcagaccaag tttactcata tatactttag attgatttac gcgccctgta gcggcgcatt
     9661 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     9721 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     9781 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     9841 caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     9901 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     9961 aacactcaac cctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggc
    10021 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
    10081 aacgtttaca atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
    10141 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
    10201 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
    10261 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
    10321 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
    10381 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtca
    10441 ggcatttgag aagcacacgg tcacactgct tccggtagtc aataaaccgg taaaccagca
    10501 atagacataa gcggctattt aacgaccctg ccctgaaccg acgaccgggt cgaatttgct
    10561 ttcgaatttc tgccattcat ccgcttatta tcacttattc aggcgtagca accaggcgtt
    10621 taagggcacc aataactgcc ttaaaaaaat tacgccccgc cctgccactc atcgcagtac
    10681 tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc
    10741 tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa
    10801 acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc
    10861 cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg
    10921 ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg
    10981 tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa
    11041 gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg gaattccgga
    11101 tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt
    11161 ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat
    11221 tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg
    11281 gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat
    11341 aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt
    11401 acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc ggtatcaaca
    11461 gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat ttattcggcg
    11521 caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt gtttttgagg
    11581 tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg acggggtggt
    11641 gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact ggcttactat
    11701 gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa aaggctgcac
    11761 cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc actgactcgc
    11821 tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc ggagatttcc
    11881 tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa agccgttttt
    11941 ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc agtggtggcg
    12001 aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc tcgtgcgctc
    12061 tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc gtttgtctca
    12121 ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac tgtatgcacg
    12181 aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
    12241 cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt agaggagtta
    12301 gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg tgactgcgct
    12361 cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt cgaaaaaccg
    12421 ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc aaaacgatct
    12481 caagaagatc atcttattaa tcagataaaa tatttctagg ctcatgagcc cgaagtggcg
    12541 agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc
    12601 gccggtgatg ccggccacga tgcgtccggc gtagaggatc tgctcatgtt tgacagctta
    12661 tc
//